7AQH

Cell wall binding domain of the Staphylococcal phage 2638A endolysin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 1
Unit cell lengths	62.130, 62.510, 66.171
Unit cell angles	111.01, 108.39, 90.18

Refinement procedure

Resolution	44.110 - 2.490
R-factor	0.2517
Rwork	0.250
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5leo
RMSD bond length	0.002
RMSD bond angle	0.469
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.110	2.570
High resolution limit [Å]	2.488	2.488
Rmerge	0.087	0.678
Rmeas	0.117	0.907
Rpim	0.077	0.598
Number of reflections	51577	2454
<I/σ(I)>	4.31	0.68
Completeness [%]	90.9	79.34
Redundancy	2.06
CC(1/2)	0.992	0.486

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	292	Protein buffer: 20 mM Tris, 150 mM NaCl, pH 7.4 Crystallization buffer: 1% (W/V) tryptone, 0.05 M HEPES, pH 7.0, 12% (w/v) PEG 3350