7AQ5
Pseudomonas stutzeri nitrous oxide reductase mutant, H583N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-13 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.369980 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.289, 76.452, 109.485 |
Unit cell angles | 90.00, 93.38, 90.00 |
Refinement procedure
Resolution | 62.647 - 1.700 |
R-factor | 0.1632 |
Rwork | 0.161 |
R-free | 0.19920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rkz |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.647 | 1.853 |
High resolution limit [Å] | 1.700 | 1.705 |
Number of reflections | 98286 | 4915 |
<I/σ(I)> | 9.9 | |
Completeness [%] | 94.4 | |
Redundancy | 6.6 | |
CC(1/2) | 0.993 | 0.383 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M bis-tris propane buffer at pH 8.5, 0.1 M sodium formate, 0.1 M sodium chloride, and 25% (w/v) of a medium molecular weight (MMW) polyethylene glycol mixture (PEG 2K, 3350, 4K and 5K MME) |