7APV

Structure of Artemis/DCLRE1C/SNM1C in complex with Ceftriaxone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 1
Unit cell lengths	35.880, 48.103, 48.252
Unit cell angles	82.76, 76.29, 86.30

Refinement procedure

Resolution	47.690 - 1.950
R-factor	0.1863
Rwork	0.184
R-free	0.22720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6tt5
RMSD bond length	0.010
RMSD bond angle	1.502
Data reduction software	DIALS
Data scaling software	Aimless (0.5.28)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.690	47.690	2.000
High resolution limit [Å]	1.950	8.940	1.950
Rmerge	0.116	0.040	0.649
Total number of observations	78348	823	5605
Number of reflections	22195	228	1569
<I/σ(I)>	8.6	25.2	2.8
Completeness [%]	98.0	97	97.2
Redundancy	3.5	3.6	3.6
CC(1/2)	0.993	0.996	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	15% PEG3350 and 0.2 M Ammonium chloride