7APM
tRNA-guanine transglycosylase H319C mutant spin-labeled with MTSL.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.911, 65.064, 70.813 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 45.680 - 1.660 |
| R-factor | 0.1412 |
| Rwork | 0.139 |
| R-free | 0.17580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p0d |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.934 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.680 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 47688 | 7302 |
| <I/σ(I)> | 10.4 | 2.1 |
| Completeness [%] | 97.6 | 92.8 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.997 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291.15 | 0.1 M MES, pH 5.5, 0.5 mM DTT, 13% PEG 8000, 10% DMSO |
