7AP5
Crystal structure of phycoerythrin from cyanobacterium Nostoc sp. WR13 contains multiple stacks of hexameric assemblies which resemble the rods of phycobilisome.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-22 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97950 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 192.759, 192.759, 524.486 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.005 - 2.131 |
| Rwork | 0.177 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nb4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.576 |
| Data reduction software | XDS |
| Data scaling software | pointless (1.11.14) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 159.070 | 2.322 |
| High resolution limit [Å] | 2.130 | 2.133 |
| Rmerge | 0.283 | 1.653 |
| Rmeas | 0.298 | 1.749 |
| Rpim | 0.093 | 0.567 |
| Number of reflections | 156067 | 7805 |
| <I/σ(I)> | 8 | 1.6 |
| Completeness [%] | 95.6 | 80.1 |
| Redundancy | 10.2 | 9.3 |
| CC(1/2) | 0.996 | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | Morpheus screen condition C9: 10% w/v PEG 20 000, 20% v/v PEG MME 550 precipitants; 0.03 M NPS (Nitrate, Phosphate & Sulfate) mix of additives; 0.01 M bicine/Trizma base pH 8.5 buffer system |
