7AP5

Crystal structure of phycoerythrin from cyanobacterium Nostoc sp. WR13 contains multiple stacks of hexameric assemblies which resemble the rods of phycobilisome.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97950
Spacegroup name	H 3 2
Unit cell lengths	192.759, 192.759, 524.486
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.005 - 2.131
Rwork	0.177
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nb4
RMSD bond length	0.010
RMSD bond angle	1.576
Data reduction software	XDS
Data scaling software	pointless (1.11.14)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	159.070	2.322
High resolution limit [Å]	2.130	2.133
Rmerge	0.283	1.653
Rmeas	0.298	1.749
Rpim	0.093	0.567
Number of reflections	156067	7805
<I/σ(I)>	8	1.6
Completeness [%]	95.6	80.1
Redundancy	10.2	9.3
CC(1/2)	0.996	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294.15	Morpheus screen condition C9: 10% w/v PEG 20 000, 20% v/v PEG MME 550 precipitants; 0.03 M NPS (Nitrate, Phosphate & Sulfate) mix of additives; 0.01 M bicine/Trizma base pH 8.5 buffer system