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7ANY

Structure of the Laspartomycin C Friulimicin-like mutant in complex with Geranyl phosphate

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsDIAMOND BEAMLINE I04
Synchrotron siteDiamond
BeamlineI04
Temperature [K]100
Detector technologyPIXEL
Collection date2019-07-18
DetectorDECTRIS EIGER2 X 16M
Wavelength(s)0.97951
Spacegroup nameP 1 21 1
Unit cell lengths40.132, 68.321, 40.132
Unit cell angles90.00, 120.00, 90.00
Refinement procedure
Resolution34.755 - 1.135
R-factor0.1613
Rwork0.160
R-free0.19210
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5o0z
RMSD bond length0.017
RMSD bond angle2.186
Data reduction softwareXDS
Data scaling softwareAimless
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0267)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]34.7551.271
High resolution limit [Å]1.1351.135
Rmerge0.0870.373
Rmeas0.1030.481
Rpim0.0550.300
Number of reflections360221799
<I/σ(I)>6.22.8
Completeness [%]85.331.6
Redundancy3.31.9
CC(1/2)0.9950.852
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.52910.2 M cadmium chloride, 40% MPD

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