7AMX
The crystal structure of gene product PA4063 from Pseudomonas aeruginosa in complex with zinc
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-07 |
Detector | DECTRIS EIGER2 X 4M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.467, 62.467, 102.076 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.210 - 2.850 |
Rwork | 0.185 |
R-free | 0.23690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ahw |
RMSD bond length | 0.017 |
RMSD bond angle | 1.686 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.470 | 62.470 | 3.000 |
High resolution limit [Å] | 2.850 | 9.010 | 2.850 |
Rmerge | 0.203 | 0.136 | 3.102 |
Rmeas | 0.212 | 0.143 | 3.249 |
Rpim | 0.062 | 0.043 | 0.962 |
Number of reflections | 5107 | 204 | 718 |
<I/σ(I)> | 12.5 | ||
Completeness [%] | 99.6 | ||
Redundancy | 20.5 | 16.1 | 20.7 |
CC(1/2) | 0.989 | 0.983 | 0.518 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | ammonium sulfate 2.3M, NaCl 2.1M |