7AMT
Structure of LuxR with DNA (activation)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-08 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9191 |
| Spacegroup name | P 41 |
| Unit cell lengths | 68.906, 68.906, 123.932 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.223 - 2.600 |
| R-factor | 0.2216 |
| Rwork | 0.220 |
| R-free | 0.24920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7amn |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.792 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.966 | 2.552 |
| High resolution limit [Å] | 2.543 | 2.543 |
| Number of reflections | 18962 | 156 |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 99.8 | |
| Redundancy | 13.7 | |
| CC(1/2) | 0.984 | 0.581 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 50mM Sodium Formate, 50mM Tris PH7.5, 22% PEG 2000MME |
