7AM1

Structure of yeast Ssd1, a pseudonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-15
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 1
Unit cell lengths	69.463, 74.003, 106.467
Unit cell angles	91.27, 91.80, 117.65

Refinement procedure

Resolution	61.460 - 1.900
R-factor	0.206
Rwork	0.205
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vnu 4pmw
RMSD bond length	0.004
RMSD bond angle	0.656
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.460	106.322	1.939
High resolution limit [Å]	1.900	4.986	1.903
Rmerge	0.062	0.029	1.101
Rmeas	0.076	0.036	1.366
Rpim	0.044	0.021	0.797
Number of reflections	144175	7972	7943
<I/σ(I)>	7.3	26.775	0.831
Completeness [%]	98.0	97.54	96.76
Redundancy	2.7	2.69	2.69
CC(1/2)	0.998	0.998	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	50 mM Tris-HCl pH 8.0, 25% PEG 400