7ALO

Structure of B*27:09/photoRL9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.91841
Spacegroup name	P 1
Unit cell lengths	45.200, 69.610, 82.540
Unit cell angles	80.24, 88.36, 89.88

Refinement procedure

Resolution	40.660 - 1.800
R-factor	0.2256
Rwork	0.223
R-free	0.26590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ib5
RMSD bond length	0.005
RMSD bond angle	1.044
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.920
High resolution limit [Å]	1.800	1.800
Rmeas	0.198	1.619
Number of reflections	89225	15619
<I/σ(I)>	5.4	1.06
Completeness [%]	97.1	96.8
Redundancy	3.7
CC(1/2)	0.990	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	291	20% (w/v) polyethylene glycol 3350, and 200 mM KSCN