7ALO
Structure of B*27:09/photoRL9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 |
Unit cell lengths | 45.200, 69.610, 82.540 |
Unit cell angles | 80.24, 88.36, 89.88 |
Refinement procedure
Resolution | 40.660 - 1.800 |
R-factor | 0.2256 |
Rwork | 0.223 |
R-free | 0.26590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ib5 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.044 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmeas | 0.198 | 1.619 |
Number of reflections | 89225 | 15619 |
<I/σ(I)> | 5.4 | 1.06 |
Completeness [%] | 97.1 | 96.8 |
Redundancy | 3.7 | |
CC(1/2) | 0.990 | 0.450 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 20% (w/v) polyethylene glycol 3350, and 200 mM KSCN |