7AL0

Crystal Structure of Heymonin, a Novel Frog-derived Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-30
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	H 3 2
Unit cell lengths	53.800, 53.800, 64.326
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.730 - 2.200
R-factor	0.2471
Rwork	0.246
R-free	0.27370
Structure solution method	AB INITIO PHASING
RMSD bond length	0.013
RMSD bond angle	1.650
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.730	2.340
High resolution limit [Å]	2.200	2.200
Number of reflections	1912	1912
<I/σ(I)>	28.83
Completeness [%]	98.0
Redundancy	6.7
CC(1/2)	1.000	0.971

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	ammonium sulphate