7AKX
Crystal structure of the viral rhodopsin OLPVR1 in P1 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.177, 56.968, 62.340 |
| Unit cell angles | 113.90, 90.01, 91.49 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.1896 |
| Rwork | 0.188 |
| R-free | 0.21320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sqg |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.066 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.1) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.160 | 40.160 | 1.630 |
| High resolution limit [Å] | 1.600 | 8.760 | 1.600 |
| Rmerge | 0.058 | 0.052 | 1.161 |
| Rmeas | 0.069 | 0.063 | 1.366 |
| Rpim | 0.036 | 0.035 | 0.714 |
| Total number of observations | 221715 | 1258 | 11145 |
| Number of reflections | 63486 | 397 | 3098 |
| <I/σ(I)> | 6.1 | 16.1 | 0.7 |
| Completeness [%] | 95.2 | 97.1 | 94.5 |
| Redundancy | 3.5 | 3.2 | 3.6 |
| CC(1/2) | 0.998 | 0.995 | 0.533 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 8.2 | 293 | 10 mM CaCl2, 10 mM MgCl2, 24% PEG 550, 100mM Tris (pH 8.2), monoolein |
