7AKW
Crystal structure of the viral rhodopsins chimera O1O2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9780 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 44.405, 103.396, 111.381 |
Unit cell angles | 90.00, 90.32, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.960 |
R-factor | 0.2041 |
Rwork | 0.202 |
R-free | 0.24580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sqg |
RMSD bond length | 0.002 |
RMSD bond angle | 1.053 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.330 | 41.330 | 2.000 |
High resolution limit [Å] | 1.960 | 8.970 | 1.960 |
Rmerge | 0.051 | 0.021 | 1.191 |
Rmeas | 0.061 | 0.025 | 1.407 |
Rpim | 0.032 | 0.013 | 0.742 |
Total number of observations | 126043 | 1293 | 8393 |
Number of reflections | 36098 | 382 | 2448 |
<I/σ(I)> | 12.2 | 51.3 | 0.9 |
Completeness [%] | 99.5 | 97.8 | 95.4 |
Redundancy | 3.5 | 3.4 | 3.4 |
CC(1/2) | 0.999 | 0.999 | 0.508 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 4.6 | 293 | 1.8 M Na2HPO4/KH2PO4 (pH 4.6) |