7AKP

Crystal structure of E. coli RNA helicase HrpA-D305A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-17
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.918
Spacegroup name	P 1 21 1
Unit cell lengths	39.894, 114.902, 94.614
Unit cell angles	90.00, 101.94, 90.00

Refinement procedure

Resolution	48.810 - 2.590
R-factor	0.2195
Rwork	0.211
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6zwx
RMSD bond length	0.003
RMSD bond angle	0.615
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.810	2.750
High resolution limit [Å]	2.590	2.590
Number of reflections	25697	4048
<I/σ(I)>	7.27
Completeness [%]	99.1
Redundancy	6.9
CC(1/2)	0.987	0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	7 % PEG 3350, 0.05 M HEPES, pH 7, 0.1 M potassium chloride, 0.01 M magnesium chloride