7AK6
Cryo-EM structure of ND6-P25L mutant respiratory complex I from Mus musculus at 3.8 A
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.82 |
| Software | RELION,UCSF Chimera,Coot,ISOLDE,PHENIX,Gctf |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
