7AJ9
The X-ray Structure of L,D-transpeptidase LdtA from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979270 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.920, 93.046, 249.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.340 - 2.350 |
| R-factor | 0.2403 |
| Rwork | 0.238 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.393 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 2.420 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rpim | 0.057 | 1.279 |
| Number of reflections | 54368 | 4391 |
| <I/σ(I)> | 8.6 | 0.8 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 7.1 | 7.3 |
| CC(1/2) | 0.997 | 0.411 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M HEPES pH 7 + 10% PEG 8000 + 10% Ethylene Glycol |
