7AI9
Structure of Ribonucleotide reductase R2 from Escherichia coli collected by rotation serial crystallography on a COC membrane at a synchrotron source
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-03 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 90.510, 90.510, 207.870 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.260 - 2.000 |
R-factor | 0.1711 |
Rwork | 0.170 |
R-free | 0.19070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mxr |
RMSD bond length | 0.009 |
RMSD bond angle | 0.983 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.260 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.197 | 11.050 |
Number of reflections | 64377 | 4762 |
<I/σ(I)> | 10.05 | 0.48 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 26.38 | 23.1 |
CC(1/2) | 0.999 | 0.183 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 34-37 % PAA 2100, 100 mM HEPES 7.0, 250-450 mM NaCl, 200 mM ammonium sulfate [and] 26% PAA 2100, 100 mM HEPES 7.0, 150 NaCl, 100 Malonate |