7AI8
Structure of Ribonucleotide reductase R2 from Escherichia coli collected by still serial crystallography on a COC membrane at a synchrotron source
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 90.350, 90.350, 208.550 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.970 - 2.100 |
R-factor | 0.1914 |
Rwork | 0.190 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mxr |
RMSD bond length | 0.007 |
RMSD bond angle | 0.831 |
Data reduction software | CrystFEL (0.7 / 0.8) |
Data scaling software | CrystFEL (0.7 / 0.8) |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.200 | 2.130 |
High resolution limit [Å] | 2.080 | 2.100 |
Number of reflections | 31083 | 1474 |
<I/σ(I)> | 10.74 | 1.74 |
Completeness [%] | 99.7 | 96.66 |
Redundancy | 201.59 | 15.2 |
CC(1/2) | 0.997 | 0.383 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 34-37 % PAA 2100, 100 mM HEPES 7.0, 250-450 mM NaCl, 200 mM ammonium sulfate [and] 26% PAA 2100, 100 mM HEPES 7.0, 150 NaCl, 100 Malonate |