7AI4
Crystal structure of the KLC1-TPR domain truncated from its nonTPR region ([A1-B6]-Delta-nonTPR fragment)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.220, 55.820, 103.570 |
| Unit cell angles | 90.00, 108.51, 90.00 |
Refinement procedure
| Resolution | 49.110 - 2.795 |
| R-factor | 0.2187 |
| Rwork | 0.217 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oj8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.110 | 2.960 |
| High resolution limit [Å] | 2.795 | 2.795 |
| Number of reflections | 16666 | 2608 |
| <I/σ(I)> | 10.17 | 0.92 |
| Completeness [%] | 99.0 | 96.7 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.998 | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 1.1 M sodium formate and 0.1 M Hepes pH 7.9 |
