7AHY
Crystal structure of Western clawed frog MDM2 RING domain homodimer
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-24 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.97997 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.559, 23.760, 55.744 |
Unit cell angles | 90.00, 101.22, 90.00 |
Refinement procedure
Resolution | 41.745 - 2.532 |
Rwork | 0.217 |
R-free | 0.27880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ah2 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.377 |
Data reduction software | xia2 |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.750 | 2.600 |
High resolution limit [Å] | 2.530 | 2.530 |
Number of reflections | 3830 | 274 |
<I/σ(I)> | 3.7 | |
Completeness [%] | 99.8 | |
Redundancy | 3.1 | |
CC(1/2) | 0.983 | 0.652 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1 M PCTP, 25 % w/v PEG 1500 |