7AHM

Low-resolution structure of the K+/H+ antiporter subunit KhtT in complex with c-di-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979260
Spacegroup name	P 42 2 2
Unit cell lengths	190.651, 190.651, 84.574
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.262 - 3.140
R-factor	0.239
Rwork	0.237
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7agv
RMSD bond length	0.002
RMSD bond angle	0.581
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	190.650	3.360
High resolution limit [Å]	3.140	3.140
Rmerge	0.175	1.698
Rmeas	0.185	1.789
Rpim	0.057	0.550
Number of reflections	26877	4922
<I/σ(I)>	8.1
Completeness [%]	96.5	99.8
Redundancy	9.7	10.1
CC(1/2)	0.990	0.714

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	100 mM Tris-HCl pH 8.5 2.2 M Ammonium sulfate