7AHK
Crystal structure of the outward-facing state of the substrate-free Na+-only bound glutamate transporter homolog GltPh
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-12 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97242 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 152.011, 152.011, 57.521 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.2133 |
Rwork | 0.211 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nww |
RMSD bond length | 0.013 |
RMSD bond angle | 1.249 |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.870 | 45.870 | 2.600 |
High resolution limit [Å] | 2.500 | 9.010 | 2.500 |
Rmerge | 0.171 | 0.021 | 1.365 |
Rmeas | 0.211 | 0.026 | 1.684 |
Rpim | 0.122 | 0.015 | 0.968 |
Total number of observations | 65714 | 1352 | 7580 |
Number of reflections | 25571 | 537 | 2905 |
<I/σ(I)> | 5.4 | 26.4 | 0.8 |
Completeness [%] | 96.4 | 88.4 | 98.5 |
Redundancy | 2.6 | 2.5 | 2.6 |
CC(1/2) | 0.990 | 0.999 | 0.250 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.2 | 293 | 1M KH2PO4/Na2HPO4, pH 5.2 |