7AHF
Dimeric structure of the catalytic domain of the human ubiquitin-conjugating enzyme UBE2S L114E varaiant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.317, 48.411, 70.127 |
Unit cell angles | 90.00, 107.07, 90.00 |
Refinement procedure
Resolution | 43.320 - 2.150 |
R-factor | 0.1936 |
Rwork | 0.191 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zdn |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.322 | 2.283 |
High resolution limit [Å] | 2.148 | 2.148 |
Rmerge | 0.045 | 0.303 |
Rmeas | 0.063 | 0.428 |
Rpim | 0.045 | 0.303 |
Number of reflections | 15974 | 1561 |
<I/σ(I)> | 11.83 | 2.47 |
Completeness [%] | 100.0 | 99.49 |
Redundancy | 2 | |
CC(1/2) | 0.998 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.33 M magnesium formate dihydrate, 15% PEG 3350 |