7AH3
Kinase domain of cSrc in complex with a pyrazolopyrimidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-09 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.260, 63.420, 74.930 |
| Unit cell angles | 101.72, 90.89, 90.01 |
Refinement procedure
| Resolution | 43.260 - 1.950 |
| R-factor | 0.1891 |
| Rwork | 0.187 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o2p |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.260 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.083 | 1.196 |
| Rmeas | 0.098 | 1.424 |
| Rpim | 0.052 | 0.762 |
| Number of reflections | 53873 | 5274 |
| <I/σ(I)> | 9.19 | |
| Completeness [%] | 97.0 | 95.13 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.997 | 0.441 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0 to 30 mM NaCl, pH 7.0 9 to 20 % ethylene glycol |
