7AH2

Crystal structure of Zebrafish MDM2 RING domain homodimer

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	46.163, 23.859, 54.306
Unit cell angles	90.00, 101.66, 90.00

Refinement procedure

Resolution	45.210 - 2.872
Rwork	0.206
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vjf
RMSD bond length	0.006
RMSD bond angle	1.429
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.185	2.922
High resolution limit [Å]	2.872	2.872
Number of reflections	2814	129
<I/σ(I)>	6.3
Completeness [%]	100.0
Redundancy	3.2
CC(1/2)	0.949	0.667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.1 M imidazole, 0.12 M monosaccharides, 37.5 % (w/v) MPD_P1K_P3350 (Morpheus, Molecular Dimensions)