7AGY
Structure of the N-domain of the K+/H+ antiporter subunit KhtT at pH 8.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978566 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.197, 63.594, 64.568 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.284 - 1.540 |
R-factor | 0.2252 |
Rwork | 0.225 |
R-free | 0.23380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7agv |
RMSD bond length | 0.006 |
RMSD bond angle | 0.983 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.310 | 1.570 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.051 | 1.048 |
Rmeas | 0.053 | 1.089 |
Rpim | 0.015 | 0.295 |
Number of reflections | 22120 | 1075 |
<I/σ(I)> | 20.7 | 1.6 |
Completeness [%] | 99.8 | |
Redundancy | 12.9 | |
CC(1/2) | 0.999 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Bis-Tris propane pH 8.5 200 mM KNa tartrate 20% PEG 3350 |