7AGV
High-resolution structure of the K+/H+ antiporter subunit KhtT in complex with c-di-AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979260 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.945, 127.598, 81.881 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 81.462 - 1.850 |
| R-factor | 0.2062 |
| Rwork | 0.204 |
| R-free | 0.24190 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | SAD structure from data collected at SOLEIL-PROXIMA1. |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 127.600 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.033 | 0.855 |
| Rmeas | 0.041 | 1.045 |
| Rpim | 0.023 | 0.595 |
| Number of reflections | 124845 | 6197 |
| <I/σ(I)> | 14.3 | 1.3 |
| Completeness [%] | 98.5 | 99.1 |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.998 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 100 mM Sodium acetate pH 4.6 260 mM Calcium chloride 15% (v/v) 2-propanol |
