7AGL

crystal structure of the apo form of the N-acetylmuramyl-L-alanine amidase, Ami1, from Mycobacterium abscessus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	85.920, 85.920, 73.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.420 - 1.600
R-factor	0.1698
Rwork	0.168
R-free	0.19590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lq6
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15rc3_3435)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.700	1.650
High resolution limit [Å]	1.600	1.600
Rmeas	0.090	1.130
Number of reflections	35745	5957
<I/σ(I)>	10.45
Completeness [%]	99.1
Redundancy	6.3
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M Bis-Tris pH 5.5, 1.6 M ammonium sulfate and, 1% (w/v) PEG 3350