7AGJ
Ribonucleotide Reductase R1 protein from Aquifex aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 108.584, 108.584, 618.107 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 94.040 - 2.700 |
| R-factor | 0.22338 |
| Rwork | 0.221 |
| R-free | 0.27216 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hnf |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.208 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 60783 | 2944 |
| <I/σ(I)> | 17.3 | |
| Completeness [%] | 99.6 | |
| Redundancy | 20 | |
| CC(1/2) | 0.996 | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate pH 4.5, 0.2 M lithium sulfate, 30 % PEG400 |
