7AG9
Structure of the Kar9 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-29 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 |
Unit cell lengths | 125.621, 125.621, 165.274 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.860 - 3.300 |
R-factor | 0.2659 |
Rwork | 0.264 |
R-free | 0.28900 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 1.204 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 38506 | 12118 |
<I/σ(I)> | 11.09 | |
Completeness [%] | 99.8 | |
Redundancy | 12.3 | |
CC(1/2) | 0.999 | 0.510 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20 mM Tris-HCl, pH 7.5, 500 mM NaCl supplemented with 250 mM gamma amino butyric acid (GABA). |