7AEX

NRD-HEPN domains (N-terminal truncation) of Escherichia coli RnlA endoribonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-10
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 2
Unit cell lengths	69.658, 81.676, 54.251
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.190 - 1.950
R-factor	0.1904
Rwork	0.186
R-free	0.23480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i80
RMSD bond length	0.007
RMSD bond angle	0.864
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.190	2.015
High resolution limit [Å]	1.946	1.946
Number of reflections	23246	1700
<I/σ(I)>	12.72	0.84
Completeness [%]	96.5	74.17
Redundancy	13.1	11.1
CC(1/2)	0.999	0.469

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	292	0.2 M NaCl; 0.1 M BIS-Tris; pH 5.5; 25 % PEG 3350