7ADT
Orf virus Apoptosis inhibitor ORFV125
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-19 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99587 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 69.511, 45.564, 105.965 |
| Unit cell angles | 90.00, 106.06, 90.00 |
Refinement procedure
| Resolution | 34.339 - 2.210 |
| R-factor | 0.234552303192 |
| Rwork | 0.233 |
| R-free | 0.25926 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.457 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.640 | 2.280 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.098 | 0.594 |
| Number of reflections | 16213 | 1395 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.990 | 0.452 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M Magnesium chloride hexahydrate, 0.1 M Bis-Tris pH 5.5, 25% PEG 3350 |
