7ADT

Orf virus Apoptosis inhibitor ORFV125

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99587
Spacegroup name	I 1 2 1
Unit cell lengths	69.511, 45.564, 105.965
Unit cell angles	90.00, 106.06, 90.00

Refinement procedure

Resolution	34.339 - 2.210
R-factor	0.234552303192
Rwork	0.233
R-free	0.25926
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.457
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.640	2.280
High resolution limit [Å]	2.210	2.210
Rmerge	0.098	0.594
Number of reflections	16213	1395
<I/σ(I)>	4.8
Completeness [%]	99.9
Redundancy	3.5
CC(1/2)	0.990	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0.2 M Magnesium chloride hexahydrate, 0.1 M Bis-Tris pH 5.5, 25% PEG 3350