7ADN
apo tRNA-guanine transglycosylase C158S/C281S/Y330C/H333A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 127.864, 127.864, 115.486 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.850 - 1.920 |
R-factor | 0.1724 |
Rwork | 0.172 |
R-free | 0.18760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.007 |
RMSD bond angle | 0.794 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.850 | 2.030 |
High resolution limit [Å] | 1.920 | 1.920 |
Number of reflections | 43128 | 6777 |
<I/σ(I)> | 31.6 | |
Completeness [%] | 99.7 | 98.5 |
Redundancy | 24.8 | 20 |
CC(1/2) | 0.999 | 0.963 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 283.15 | 0.1 M magnesium formate |