7ADA
Crystal structure of helicase Pif1 from Thermus oshimai mutant Q164C-E409C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978910 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.070, 58.679, 118.155 |
Unit cell angles | 90.00, 92.21, 90.00 |
Refinement procedure
Resolution | 52.550 - 3.340 |
R-factor | 0.2477 |
Rwork | 0.245 |
R-free | 0.30160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6s3e |
RMSD bond length | 0.004 |
RMSD bond angle | 0.877 |
Data reduction software | XDS (Mar 15, 2019, built on 20191211) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.550 | 3.460 |
High resolution limit [Å] | 3.340 | 3.340 |
Rmerge | 0.049 | 0.600 |
Rmeas | 0.054 | 0.647 |
Rpim | 0.021 | 0.241 |
Number of reflections | 15002 | 1501 |
<I/σ(I)> | 24.54 | 2.79 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 6.9 | 7.1 |
CC(1/2) | 0.998 | 0.922 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 0.1 M Sodium Hepes-MOPS (pH 7.5) 0.03 M CaCl2 0.03 M MgCl2 10% PEG8000 20% ethylene glycol. |