7ACZ
RdeltaD2 H/L (LMW SLP/HMW SLP) complex from C. difficile SlpA (R20291 strain)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-28 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 1 |
Unit cell lengths | 52.745, 80.352, 81.920 |
Unit cell angles | 97.03, 90.22, 90.22 |
Refinement procedure
Resolution | 52.740 - 3.500 |
R-factor | 0.2557 |
Rwork | 0.254 |
R-free | 0.27860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Dep D_19292110995 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.595 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.740 | 3.630 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.510 | 0.904 |
Rmeas | 0.607 | 1.069 |
Rpim | 0.324 | 0.563 |
Number of reflections | 16691 | 1620 |
<I/σ(I)> | 10 | 2.47 |
Completeness [%] | 98.8 | 97.03 |
Redundancy | 3.4 | 3.5 |
CC(1/2) | 0.616 | 0.438 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | 0.1 M MES pH 6.0 0.875 M Lithium chloride 11.5% PEG 6,000 10% Glycerol |