7ACY

H/L (SLPH/SLPL) complex from C. difficile (CD630 strain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 1
Unit cell lengths	72.670, 78.250, 81.580
Unit cell angles	81.89, 66.98, 65.26

Refinement procedure

Resolution	52.310 - 2.550
R-factor	0.23
Rwork	0.230
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cvz 5j6q D_129211090/992/994
RMSD bond length	0.004
RMSD bond angle	0.916
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.310	2.640
High resolution limit [Å]	2.550	2.550
Rmerge	0.128	0.704
Rmeas	0.153	0.836
Rpim	0.083	0.445
Number of reflections	47230	4750
<I/σ(I)>	7	1.5
Completeness [%]	96.4	97.6
Redundancy	3.3	3.4
CC(1/2)	0.992	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	0.1 M MES pH 6.2 1.25 Lithium chloride 16% PEG 6000 10% Glycerol