7ACW

LID/HID (LMW SLP and HMW SLP interacting domains) from C. difficile (R7404 strain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	73.273, 56.705, 61.813
Unit cell angles	90.00, 122.71, 90.00

Refinement procedure

Resolution	52.010 - 1.500
R-factor	0.166
Rwork	0.164
R-free	0.20450
Structure solution method	AB INITIO PHASING
RMSD bond length	0.008
RMSD bond angle	1.146
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	Arcimboldo
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.010	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.047	0.220
Rmeas	0.055	0.270
Rpim	0.028	0.154
Number of reflections	34063	3318
<I/σ(I)>	8.1
Completeness [%]	98.9	95.3
Redundancy	3.4	2.6
CC(1/2)	0.998	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.6M sodium citrate tribasic dihidrate