7ACV

SLPL/HID (LMW SLP complex with HMW SLP interacting domain - HID) from C. difficile (R7404 strain)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.969
Spacegroup name	C 1 2 1
Unit cell lengths	172.870, 29.446, 144.329
Unit cell angles	90.00, 94.22, 90.00

Refinement procedure

Resolution	86.200 - 2.400
R-factor	0.2535
Rwork	0.251
R-free	0.30120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cvz D_1292110990
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.350	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	29460	2924
<I/σ(I)>	5.48	1.9
Completeness [%]	99.5
Redundancy	5.1
CC(1/2)	0.979	0.713

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium sulphate, 0.1 M MES pH6.5, 35 % MPD