7ACL

Crystal structure of E. coli HTH-type transcriptional regulator RcdA at 2.05 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	39.595, 76.608, 105.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.357 - 2.049
R-factor	0.1883
Rwork	0.185
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5x5i
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.357	43.357	2.170
High resolution limit [Å]	2.049	6.090	2.050
Rmerge	0.090	0.036	0.954
Rmeas	0.093	0.038	1.000
Number of reflections	20613	886	3131
<I/σ(I)>	18.58	58.34	2.11
Completeness [%]	98.9	99	94.9
Redundancy	12.624	11.633	10.864
CC(1/2)	0.999	0.999	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M magnesium formate, 15% PEG3350