7ACI

In meso structure of apolipoprotein N-acyltransferase, Lnt, from Escherichia coli in 9.8 monoacylglycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.999
Spacegroup name	P 21 2 21
Unit cell lengths	48.775, 76.173, 156.531
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.570 - 2.300
R-factor	0.2167
Rwork	0.214
R-free	0.25790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n6h
RMSD bond length	0.004
RMSD bond angle	0.635
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.570	2.382
High resolution limit [Å]	2.300	2.300
Number of reflections	26629	2578
<I/σ(I)>	7.5
Completeness [%]	99.3
Redundancy	12.9
CC(1/2)	0.990	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	0.1M MES pH 6.0, * %(v/v) MPD, 0.05-0.4 M sodium thiocyanate