7AC4
Structure of insulin collected by rotation serial crystallography on a COC membrane at a synchrotron source
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 77.960, 77.960, 77.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.980 - 1.460 |
| R-factor | 0.1735 |
| Rwork | 0.173 |
| R-free | 0.18000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d53 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.980 | 1.500 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Number of reflections | 13869 | 1379 |
| <I/σ(I)> | 8.26 | 0.7 |
| Completeness [%] | 99.9 | |
| Redundancy | 7.16 | |
| CC(1/2) | 0.997 | 0.188 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50mM Na2HPO4, 10mM EDTA pH 10.8 |
