7AC3

Structure of thaumatin collected by rotation serial crystallography on a COC membrane at a synchrotron source

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	58.000, 58.000, 150.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.916 - 1.650
R-factor	0.1677
Rwork	0.165
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4axr
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.920	1.690
High resolution limit [Å]	1.650	1.650
Number of reflections	31814	3127
<I/σ(I)>	4.19	0.54
Completeness [%]	99.5
Redundancy	7.98
CC(1/2)	0.986	0.144

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	1.6 M potassium sodium tartrate tetrahydrate, 100mM Na Hepes pH 7.0