7AB4
Crystal structure of the Escherichia coli toxin-antitoxin system HipBST (HipT S59A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.54180 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 285.257, 107.146, 58.449 |
Unit cell angles | 90.00, 90.71, 90.00 |
Refinement procedure
Resolution | 50.590 - 3.340 |
R-factor | 0.2041 |
Rwork | 0.202 |
R-free | 0.24350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PBD-code from depostion entry D_1292109701 |
Data reduction software | XDS (VERSION Mar 15, 2019 BUILT=20190315) |
Data scaling software | XSCALE (VERSION Mar 15, 2019 BUILT=20190315) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.19rc5_4047) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.590 | 50.590 | 3.320 |
High resolution limit [Å] | 3.240 | 14.490 | 3.240 |
Rmerge | 0.332 | 0.049 | 3.316 |
Rmeas | 0.381 | 0.056 | 3.807 |
Total number of observations | 184956 | ||
Number of reflections | 45019 | 456 | 3141 |
<I/σ(I)> | 4.73 | 19.48 | 0.55 |
Completeness [%] | 81.9 | 75.1 | 78.4 |
Redundancy | 4.108 | 3.943 | 4.097 |
CC(1/2) | 0.983 | 0.996 | 0.167 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293.15 | 0.1M BICINE, 6% MPD |