7AAQ
sugar/H+ symporter STP10 in outward occluded conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97942 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.496, 92.780, 119.788 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.390 - 1.810 |
R-factor | 0.1887 |
Rwork | 0.187 |
R-free | 0.21200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h7d |
Data reduction software | DIALS (1-14-4) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.900 | 59.930 | 1.840 |
High resolution limit [Å] | 1.810 | 4.910 | 1.810 |
Rmerge | 0.168 | 0.047 | 2.501 |
Rmeas | 0.176 | 0.050 | 2.490 |
Rpim | 0.053 | 0.015 | 0.788 |
Total number of observations | 665695 | 7411 | 47580 |
Number of reflections | 61268 | 3317 | 2964 |
<I/σ(I)> | 7.7 | 25.2 | 1.1 |
Completeness [%] | 100.0 | 100 | 98.7 |
Redundancy | 11 | 22.2 | 1 |
CC(1/2) | 0.998 | 0.999 | 0.651 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 4 | 292.15 | 0.1-0.15M Ammonium Acetate, 0.1M Sodium Citrate and 36-40% PEG400 |