7AAH

Structure of human pERp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96863
Spacegroup name	P 21 21 21
Unit cell lengths	63.714, 63.909, 71.889
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.764 - 1.400
R-factor	0.1487
Rwork	0.147
R-free	0.18570
Structure solution method	AB INITIO PHASING
RMSD bond length	0.008
RMSD bond angle	0.964
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	58099	5426
<I/σ(I)>	12.88
Completeness [%]	99.1
Redundancy	6.2
CC(1/2)	0.999	0.364

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	Lithium citrate tribasic tetrahydrate, Glycerol ethoxylate, n-decyl-beta-D-maltopyranoside