7AAE
Crystal structure of Human serum albumin in complex with myristic acid at 2.27 Angstrom Resolution
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 95.315, 38.537, 184.280 |
| Unit cell angles | 90.00, 104.45, 90.00 |
Refinement procedure
| Resolution | 29.228 - 2.270 |
| Rwork | 0.224 |
| R-free | 0.29070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.859 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.230 | 2.350 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.060 | |
| Number of reflections | 30458 | 2962 |
| <I/σ(I)> | 15.28 | 2.1 |
| Completeness [%] | 99.5 | |
| Redundancy | 5.5 | |
| CC(1/2) | 0.999 | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 50 mM HEPES, 50 mM MOPS, 30 mM sodium fluoride, 30 mM sodium bromide, 30 mM sodium iodide, 12.5% v/v MPD, 12.5% w/v PEG 1000, 12.5% w/v PEG 3350 |
