7AA8

Structure of SCOC LIR bound to GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97
Spacegroup name	P 32 2 1
Unit cell lengths	52.820, 52.820, 81.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.740 - 1.250
R-factor	0.1694
Rwork	0.168
R-free	0.19390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hyo
RMSD bond length	0.005
RMSD bond angle	0.787
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.740	1.290
High resolution limit [Å]	1.250	1.250
Rmerge	0.040	2.000
Rpim	0.010	0.700
Number of reflections	37099	3670
<I/σ(I)>	15.85
Completeness [%]	99.8
Redundancy	7.8
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.4	293	2.9% PEG 550 MME, 50 mM Bicine pH 8.4, 30% PEG 1500