7AA7

Structure of SCOC pS12/pS18 LIR motif bound to GABARAPL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-21
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97
Spacegroup name	P 1
Unit cell lengths	28.364, 37.169, 62.482
Unit cell angles	81.75, 89.60, 67.82

Refinement procedure

Resolution	31.920 - 1.450
R-factor	0.1528
Rwork	0.151
R-free	0.18940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hoi
RMSD bond length	0.010
RMSD bond angle	1.123
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.920	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.090	0.600
Rpim	0.080	0.600
Number of reflections	41242	2051
<I/σ(I)>	15.1
Completeness [%]	99.5
Redundancy	3
CC(1/2)	0.980	0.460

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1M MES pH 6.5, 3.5M AMSO4, 1% MPD