7AA1
Structural comparison of cellular retinoic acid binding proteins I and II in the presence and absence of natural and synthetic ligands
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2019-04-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 21 3 |
Unit cell lengths | 78.560, 78.560, 78.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.398 - 1.710 |
Rwork | 0.180 |
R-free | 0.20110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5obg |
RMSD bond length | 0.012 |
RMSD bond angle | 1.754 |
Data reduction software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.398 | 45.360 | 1.740 |
High resolution limit [Å] | 1.710 | 9.050 | 1.710 |
Rmerge | 0.077 | 0.036 | 2.227 |
Rmeas | 0.082 | 0.038 | 2.356 |
Rpim | 0.026 | 0.013 | 0.760 |
Number of reflections | 17775 | 2123 | 17213 |
<I/σ(I)> | 18.8 | ||
Completeness [%] | 100.0 | ||
Redundancy | 18.3 | 14.3 | 18.3 |
CC(1/2) | 0.999 | 0.999 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG based vapor diffusion |