7A9Z

Structural comparison of cellular retinoic acid binding protein I and II in the presence and absence of natural and synthetic ligands

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	120
Detector technology	PIXEL
Collection date	2020-01-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 31 2 1
Unit cell lengths	127.545, 127.545, 52.161
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	110.457 - 2.410
Rwork	0.205
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cbr
RMSD bond length	0.009
RMSD bond angle	1.559
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.460	2.500
High resolution limit [Å]	2.410	2.410
Rmerge	0.107	6.347
Rmeas	0.392	6.751
Rpim	0.123	2.287
Number of reflections	19102	33328
<I/σ(I)>	7.1
Completeness [%]	99.9
Redundancy	19.2	16.8
CC(1/2)	0.994	0.398

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	PEG mixture